Journal: Scientific Reports

Article Title: Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue

doi: 10.1038/srep45577

Figure Lengend Snippet: ( a – c ) The number of cells after dinaciclib treatment. Cell number in 49 fields (7 × 7) was calculated and shown in the graph (n = 4). ( a ) Total cell number. **p < 0.01 vs control. ( b ) Number of OCT4 positive cells. **p < 0.01 vs control. ( c ) Number of OCT4 negative cells. n.s., not significant. ( d ) The percentage of OCT4 positive cells. **p < 0.01 vs control. *p < 0.05 vs 6 nM. ( e ) Representative images of OCT4 positive cells (red). Nuclei were stained with Hoechst (blue). Scale bars = 200 μm.

Article Snippet: For immunocytochemistry: mouse monoclonal anti-cardiac troponin T antibody (cTnT; Thermo Scientific, Rockford, IL, USA), goat polyclonal anti-OCT4 antibody (R&D systems, Minneapolis, MN, USA), rabbit polyclonal anti-Ki67 antibody (Abcam, Cambridge, UK).

Techniques: Control, Staining

Journal: Cell Stem Cell

Article Title: Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs

doi: 10.1016/j.stem.2019.03.012

Figure Lengend Snippet:

Article Snippet: anti-OCT4 - Rabbit polyclonal , Abcam , Abcam Cat# ab19857; RRID: AB_445175 ).

Techniques: Virus, Plasmid Preparation, Recombinant, ChIP-sequencing, RNA Sequencing, Derivative Assay, Software, ChIA Pet Assay

Journal: Biology of reproduction

Article Title: Deletion of Mylk1 in oocytes causes delayed morula-to-blastocyst transition and reduced fertility without affecting folliculogenesis and oocyte maturation in mice.

doi: 10.1095/biolreprod.114.122127

Figure Lengend Snippet: FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for Oct4 (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/

Article Snippet: Antibodies used in our experiments were available from the following companies: rabbit monoclonal anti-MLCK antibody (Abcam); rabbit polyclonal anti-phospho-myosin light chain 2 (pMLC2) (Ser19) antibody (CST); mouse monoclonal anti-b-actin antibody (Santa Cruz); goat polyclonal anti-Oct4 antibody (Santa Cruz); rabbit polyclonal anti-Bub3 antibody (Santa Cruz); rabbit monoclonal anti-a-tubulin antibody (CST); fluorescein isothiocyanate (FITC)-conjugated rabbit anti-goat immunoglobulin G (IgG) and FITCconjugated goat anti-rabbit IgG (Zhongshan Golden Bridge Biotechnology); and Cy5-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Laboratory).

Techniques: Mutagenesis, Control, Cell Culture, In Vitro, Staining, Microscopy